Anasir Mohd IshtiaqKamel KhayriAdypatti Nor MalizzaJamaluddin Mohammad SyafiqAhmad Farah AmiraNorhisham Siti NurhidayahSukri Muhammad Zulfazli MohamadRosli Nur RafiqahSaif Siti NorazrinaBasarudin Nurul IzzatiAzzam-Sayuti MohamadKarim Akmal Hayat AbdulPuat Mahirah KamilThayan RavindranZain Rozainanee Mohd2025-09-172025-3-3110.5365/wpsar.2025.16.1.1140https://pubs.cidrz.org/handle/123456789/11722<jats:p>This report describes an approach to recover SARS-CoV-2 RNA from rapid antigen test kit (RTK-antigen) cassettes for whole-genome sequencing (WGS). RNA samples were recovered from 33 RTK-antigen cassettes for WGS, with 18 samples achieving more than 80% genome coverage. This work provides a proof-of-concept that positive RTK-antigen cassettes can be safely transported, stored and subjected to WGS, enabling swift identification of circulating variants.</jats:p>Whole-genome sequencing of SARS-CoV-2 from residual viral RNA present on positive rapid antigen test kits for genomic surveillancehttps://doi.org/10.5365/wpsar.2025.16.1.1140