Browsing by Author "Muleya W"

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    Characterization of human respiratory syncytial virus in children with severe acute respiratory infection before and during the COVID-19 pandemic.
    (2024-Jun) Simusika P; Okamoto M; Dapat C; Muleya W; Malisheni M; Azam S; Imamura T; Saito M; Mwape I; Mpabalwani E; Monze M; Oshitani H; Tohoku University Graduate School of Medicine, Department of Virology, Sendai, Japan.; Center for Infectious Disease Research in Zambia, Lusaka, Zambia.; University Teaching Hospitals, Pathology and Microbiology Department, Virology Laboratory, Lusaka, Zambia.; University of Zambia, School of Veterinary Medicine, Department of Biomedical Sciences, Lusaka, Zambia.; University of Zambia, School of Medicine, Department of Pediatrics and Child Health, Lusaka, Zambia.; Levy Mwanawasa Medical University, Institute of Basic and Biomedical Sciences ,Lusaka, Zambia.; CIDRZ; Centre for Infectious Disease Research in Zambia (CIDRZ)
    OBJECTIVES: Annual outbreaks of human respiratory syncytial virus (HRSV) are caused by newly introduced and locally persistent strains. During the COVID-19 pandemic, global and local circulation of HRSV significantly decreased. This study was conducted to characterize HRSV in 2018-2022 and to analyze the impact of COVID-19 on the evolution of HRSV. DESIGN/METHODS: Combined oropharyngeal and nasopharyngeal swabs were collected from children hospitalized with severe acute respiratory infection at two hospitals in Zambia. The second hypervariable region of the attachment gene G was targeted for phylogenetic analysis. RESULTS: Of 3113 specimens, 504 (16.2%) were positive for HRSV, of which 131 (26.0%) and 66 (13.1%) were identified as HRSVA and HRSVB, respectively. In early 2021, an increase in HRSV was detected, caused by multiple distinct clades of HRSVA and HRSVB. Some were newly introduced, whereas others resulted from local persistence. CONCLUSIONS: This study provides insights into the evolution of HRSV, driven by global and local circulation. The COVID-19 pandemic had a temporal impact on the evolution pattern of HRSV. Understanding the evolution of HRSV is vital for developing strategies for its control.
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    Molecular Detection and Characterization of
    (2021) Chitanga S; Chibesa K; Sichibalo K; Mubemba B; Nalubamba KS; Muleya W; Changula K; Simulundu E; Centre for Infectious Diseases Research in Zambia, Lusaka, Zambia.; Department of Biomedical Sciences, School of Health Sciences, University of Zambia, Lusaka, Zambia.; Department of Pathobiology, School of Veterinary Medicine, University of Namibia, Windhoek, Namibia.; Department of Biomedical Sciences, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.; Department of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.; Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.; Department of Biomedical Sciences, School of Medicine, Copperbelt University, Ndola, Zambia.; Department of Paraclinical Studies, School of Veterinary Sciences, University of Zambia, Lusaka, Zambia.; Macha Research Trust, Choma, Zambia.; School of Life Sciences, College of Agriculture, Engineering and Sciences, University of KwaZulu-Natal, Durban, South Africa.; Department of Wildlife Sciences, School of Natural Resources, Copperbelt University, Kitwe, Zambia.; CIDRZ; Centre for Infectious Disease Research in Zambia (CIDRZ)
    Tick-borne zoonotic pathogens are increasingly becoming important across the world. In sub-Saharan Africa, tick-borne pathogens identified include viruses, bacteria and protozoa, with
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    Mosquito-Borne Viral Pathogens Detected in Zambia: A Systematic Review.
    (2021-Aug-10) Velu RM; Kwenda G; Libonda L; Chisenga CC; Flavien BN; Chilyabanyama ON; Simunyandi M; Bosomprah S; Sande NC; Changula K; Muleya W; Mburu MM; Mubemba B; Chitanga S; Tembo J; Bates M; Kapata N; Orba Y; Kajihara M; Takada A; Sawa H; Chilengi R; Simulundu E; HerpeZ Infection Research and Training, University Teaching Hospital, Lusaka Private Bag RW1X Ridgeway, Lusaka P.O. Box 10101, Zambia.; Centre for Infectious Disease Research in Zambia, Lusaka P.O. Box 34681, Zambia.; School of Life Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, South Africa.; School of Life Sciences, University of Lincoln, Brayford Pool, Lincoln LN6 7TS, UK.; Department of Paraclinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka P.O. Box 32379, Zambia.; Macha Research Trust, Choma P.O. Box 630166, Zambia.; Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, N 20 W10, Kita-ku, Sapporo 001-0020, Japan.; Department of Biomedical Sciences, School of Veterinary Medicine, University of Zambia, Lusaka P.O. Box 32379, Zambia.; Department of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka P.O. Box 32379, Zambia.; Division of Global Epidemiology, International Institute for Zoonosis Control, Hokkaido University, N 20 W10, Kita-ku, Sapporo 001-0020, Japan.; School of Veterinary Medicine, University of Namibia, Windhoek Private Bag 13301, Namibia.; Department of Biostatistics, School of Public Health, University of Ghana, Accra P.O. Box LG13, Ghana.; Africa Center of Excellence for Infectious Diseases of Humans and Animals, University of Zambia, Lusaka P.O. Box 32379, Zambia.; Department of Zoology and Aquatic Sciences, School of Natural Resources, Copperbelt University, Kitwe P.O. Box 21692, Zambia.; Global Virus Network, 725 W Lombard St., Baltimore, MD 21201, USA.; Zambia National Public Health Institute, Ministry of Health, Lusaka P.O. Box 30205, Zambia.; Department of Disease Control and Prevention, School of Medicine and Health Sciences, Eden University, Lusaka P.O. Box 37727, Zambia.; Department of Biomedical Sciences, School of Health Sciences, University of Zambia, Lusaka P.O. Box 50110, Zambia.; CIDRZ; Centre for Infectious Disease Research in Zambia (CIDRZ)
    Emerging and re-emerging mosquito-borne viral diseases are a threat to global health. This systematic review aimed to investigate the available evidence of mosquito-borne viral pathogens reported in Zambia. A search of literature was conducted in PubMed and Google Scholar for articles published from 1 January 1930 to 30 June 2020 using a combination of keywords. Eight mosquito-borne viruses belonging to three families,