Characterization of Rotavirus Strains Responsible for Breakthrough Diarrheal Diseases among Zambian Children Using Whole Genome Sequencing.

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dc.contributor.affiliationDepartment of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK.
dc.contributor.affiliationInstitute of Basic and Biomedical Sciences, Levy Mwanawasa Medical University, Lusaka 10101, Zambia.
dc.contributor.affiliationDivision of Medical Virology, Faculty of Medicine and Health Sciences, Stellenbosch University, P.O. Box 241, Cape Town 8000, South Africa.
dc.contributor.affiliationInfluenza Research Institute, University of Wisconsin-Madison, Madison, WI 53706-13380, USA.
dc.contributor.affiliationUniversity Teaching Hospitals, Lusaka 10101, Zambia.
dc.contributor.affiliationEnteric Disease and Vaccine Research Unit, Centre for Infectious Disease Research in Zambia, Lusaka P.O. Box 34681, Zambia.
dc.contributor.affiliationDivision of Medical Virology, School of Pathology, Faculty of Health Sciences, University of the Free State, Bloemfontein P.O. Box 339, South Africa.
dc.contributor.affiliationCIDRZ
dc.contributor.affiliationCentre for Infectious Disease Research in Zambia (CIDRZ)
dc.contributor.authorMwape I
dc.contributor.authorLaban NM
dc.contributor.authorChibesa K
dc.contributor.authorMoono A
dc.contributor.authorSilwamba S
dc.contributor.authorMalisheni MM
dc.contributor.authorChisenga C
dc.contributor.authorChauwa A
dc.contributor.authorSimusika P
dc.contributor.authorPhiri M
dc.contributor.authorSimuyandi M
dc.contributor.authorChilengi R
dc.contributor.authorDe Beer C
dc.contributor.authorOjok D
dc.date.accessioned2025-05-23T11:43:24Z
dc.date.issued2023-Nov-26
dc.description.abstractThe occurrence of rotavirus (RV) infection among vaccinated children in high-burden settings poses a threat to further disease burden reduction. Genetically altered viruses have the potential to evade both natural infection and vaccine-induced immune responses, leading to diarrheal diseases among vaccinated children. Studies characterizing RV strains responsible for breakthrough infections in resource-limited countries where RV-associated diarrheal diseases are endemic are limited. We aimed to characterize RV strains detected in fully vaccinated children residing in Zambia using next-generation sequencing. We conducted whole genome sequencing on Illumina MiSeq. Whole genome assembly was performed using Geneious Prime 2023.1.2. A total of 76 diarrheal stool specimens were screened for RV, and 4/76 (5.2%) were RV-positive. Whole genome analysis revealed RVA/Human-wt/ZMB/CIDRZ-RV2088/2020/
dc.identifier.doi10.3390/vaccines11121759
dc.identifier.urihttps://pubs.cidrz.org/handle/123456789/10813
dc.sourceVaccines
dc.titleCharacterization of Rotavirus Strains Responsible for Breakthrough Diarrheal Diseases among Zambian Children Using Whole Genome Sequencing.

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