Whole-genome sequencing of SARS-CoV-2 from residual viral RNA present on positive rapid antigen test kits for genomic surveillance

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dc.contributor.authorAnasir Mohd Ishtiaq
dc.contributor.authorKamel Khayri
dc.contributor.authorAdypatti Nor Malizza
dc.contributor.authorJamaluddin Mohammad Syafiq
dc.contributor.authorAhmad Farah Amira
dc.contributor.authorNorhisham Siti Nurhidayah
dc.contributor.authorSukri Muhammad Zulfazli Mohamad
dc.contributor.authorRosli Nur Rafiqah
dc.contributor.authorSaif Siti Norazrina
dc.contributor.authorBasarudin Nurul Izzati
dc.contributor.authorAzzam-Sayuti Mohamad
dc.contributor.authorKarim Akmal Hayat Abdul
dc.contributor.authorPuat Mahirah Kamil
dc.contributor.authorThayan Ravindran
dc.contributor.authorZain Rozainanee Mohd
dc.date.accessioned2025-09-17T10:25:18Z
dc.date.issued2025-3-31
dc.description.abstract<jats:p>This report describes an approach to recover SARS-CoV-2 RNA from rapid antigen test kit (RTK-antigen) cassettes for whole-genome sequencing (WGS). RNA samples were recovered from 33 RTK-antigen cassettes for WGS, with 18 samples achieving more than 80% genome coverage. This work provides a proof-of-concept that positive RTK-antigen cassettes can be safely transported, stored and subjected to WGS, enabling swift identification of circulating variants.</jats:p>
dc.identifier.doi10.5365/wpsar.2025.16.1.1140
dc.identifier.urihttps://pubs.cidrz.org/handle/123456789/11722
dc.identifier.uri.pubmedhttps://doi.org/10.5365/wpsar.2025.16.1.1140
dc.sourceWestern Pacific Surveillance and Response Journal
dc.titleWhole-genome sequencing of SARS-CoV-2 from residual viral RNA present on positive rapid antigen test kits for genomic surveillance

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