Antimicrobial Potential of Combretum molle Leaf Extracts: Insights from Zambia

Abstract

<jats:p>The global rise in antimicrobial resistance (AMR) necessitates innovative solutions. This study investigates the antimicrobial activity and phytochemical composition of Combretum molle, a native Zambian plant, to explore its potential as a cost-effective source for novel antimicrobial agents. Exhaustive extraction using solvents of varying polarities was performed on dried leaf extracts of C. molle. These extracts were tested against a panel of clinically significant gram-negative and gram-positive bacterial isolates and screened for phytochemicals. Phytochemical screening revealed the presence of steroids, terpenoids, phenols, flavonoids, tannins, saponins, alkaloids, and glycosides. Antimicrobial activity was assessed using disk diffusion and broth microdilution techniques, with minimum inhibitory concentrations (MICs) determined through spectrophotometry and spot inoculation. The results revealed substantial antimicrobial activity, with hexane extracts showing the highest efficacy (MIC range: 3.6 to 50.4 mg/ml) and ethanol extracts exhibiting comparable activity to tetracycline (inhibition zones: 6 to 26 mm, P-value &lt; 0.05). These findings highlight the therapeutic potential of C. molle extracts and support their possible integration into ethnomedicine as alternatives to conventional antimicrobials. By tapping into nature's arsenal, this study contributes to the search for effective strategies against multi-drug resistance, offering hope in the fight against AMR.</jats:p>